Phlebotomy Technician Practice Exam (NCPT/CPT) 2025 – 400 Free Practice Questions to Pass the Exam

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When should a vacutainer/collection tube be mixed?

Immediately after drawing blood

within 30sec of drawing blood

After drawing blood, the vacutainer/collection tube should be mixed within 30 seconds. This agitation ensures that the blood and any anticoagulant or additive are properly mixed, preventing clotting or other issues with the sample. Mixing immediately or waiting too long after drawing can cause inaccurate or unreliable results. Therefore, options A, C and D are incorrect as they do not align with proper blood handling and can lead to compromised samples.

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After 1 minute of drawing blood

Mixing is not required

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